20 October 2004
Hypothesis: Sequencing is evil when you have to do it on gels.
Theory: pouring gels that are 0.2mm thick can really tricky as there cannot be any bubbles in it. Also, the gels do not always polymerize correctly or totally.
Proof:
I have 80 patient samples to sequence.
Each sample gets amplified with 8 different primers (80*8=640)
The most samples that I can run on one gel: 96.
The number of gels required to finish all of my samples is: 6.66
666 is the number of the devil.
Thus sequencing on an ABI377 is evil and this is why we need to get a new sequencing machine.
Theory: pouring gels that are 0.2mm thick can really tricky as there cannot be any bubbles in it. Also, the gels do not always polymerize correctly or totally.
Proof:
I have 80 patient samples to sequence.
Each sample gets amplified with 8 different primers (80*8=640)
The most samples that I can run on one gel: 96.
The number of gels required to finish all of my samples is: 6.66
666 is the number of the devil.
Thus sequencing on an ABI377 is evil and this is why we need to get a new sequencing machine.
