Life in lessons....

it is one of those day. The weather is icky and cold. This weekend is Halloween, and I am planning on going to a party on Saturday night. Sean (who is hosting the party with his roomies and is from my college days) says that they are going to be having a game of flip cup to end all flip cup games...this could quickly get out of hand, you know?

"95% of Americans get married at least one time Sandi!" Abigail

How come it seems like they all get married at the same time?

More wedding drama to come....

it is just one brick wall, after another brick wall...

it has been one of those days...for a few days now and I would like to say that it cannot get any worse, but as we all know, as soon as you say that it does. Damned it do, and damned if you do not...life is just unfair sometimes and it can really suck, you know...

As I opened up my e-mail this morning I saw that the higher powers sent out a mass e-mail about a dedication. As I am on someone else computer, the font is much smaller that I have on my own computer, I ended up reading the title as Guilt Dedication. As I was thinking that they were talking about every persons guilt being laid down for all to view, i thought that I was losing it! No, the title was quilt dedication...it is amazing to see what a difference one letter makes!

Weekend up-date: it was over all a good weekend. Nothing bad, as far as I know right now. Interesting and unexpected would be the best way to describe it. Oh and the house is all nice and clean now!

Hypothesis: Sequencing is evil when you have to do it on gels.
Theory: pouring gels that are 0.2mm thick can really tricky as there cannot be any bubbles in it. Also, the gels do not always polymerize correctly or totally.
Proof:
I have 80 patient samples to sequence.
Each sample gets amplified with 8 different primers (80*8=640)
The most samples that I can run on one gel: 96.
The number of gels required to finish all of my samples is: 6.66
666 is the number of the devil.
Thus sequencing on an ABI377 is evil and this is why we need to get a new sequencing machine.

Thanks to Jen over at LJ!

I am a hybrid of: Girl Next Door Progressive Girl Click on the pictures below to read more: Take the 'What Kind of Girl Are You?' quiz at CookingToHookup.com

This past weekend was wonderfully uneventful for the most part. Friday after I got home, I hung out with Becky for coffee and shopping. We ended up shopping at some of the most unlikely stores – Rite Aid, CVS and Giant Food. I can only speak for myself, but I enjoyed our evening together!
Saturday I went over to Kevin’s for tea and cake, ran into an ex – but that is another story for another time - came into the lab for a bit and then ran into a roomie from college which was nice. She and I had some dinner and just caught up. She is now living in the apartment that Dave and I lived in together – it was very odd to see how different it looks. It was also sad for me to find out that they totally re-did the kitchen and Dave’s bathroom and both are much nicer then the ones that we had.
Sunday was a day of rest. My Father called and informed me that as of tonight I will be car-less again. I have no problem with him taking back HIS car, but a bit of notice would have been helpful as I now have to figure out how to get to a doctor appointment on Tuesday morning. Oh well, back to the cells as things are busy here…

You Know You're From Philadelphia When...

You punctuate every sentence with, "You know" at least twice. You want olive oil, not mayonnaise on your "hoagie". You hate the Redskins You hate Dallas. You realize that your favorite dessert is "wooder ice". You find yourself using "yo" and "youse guys" when talking long-distance to family members. You know how to spell Schuylkill. You pronounce ACME "ACK-A-ME". You think that $2,500 a year for insurance on a 1977 Toyota Corolla is a bargain. You find youself at a nice restaurant thinking "I wonder if they have cheese steaks?" You sleep soundly through gunfire and ambulance sirens. You visit New York and are impressed by how clean it is. You can't eat french fries without Cheese Whiz. You call sprinkles on top of your ice cream cone "jimmies". You don't think Wawa sounds funny. You snub a cheese steak that isn't on an Amoroso roll. Your parents, brothers, sisters, aunts and uncles all live on the same block. You know who Jim O'Brien is and how he died. You can't imagine lunch without a Tastycake. You're still not sure about Jerry Penacolli. A vacation at the Jersey shore (pronounced "Down the shoore") is better than going to an island (there's more stuff to do, plus you know everybody.) You know where to find the Rocky statue. You know that only tourists go to Geno's, Pat's and Jim's for authentic cheese steaks. You only go if you're drunk and it's 3:00 a.m. You can make a cheese steak and you've never been taught You've never been to the Liberty Bell, or the only time you were there was on a class trip in third grade. You know what and where "Boathouse Row" is You will buy a pretzel from anyone, anywhere without even thinking of where it was - or where his hands have been. You can't imagine a breakfast without scrapple. You don't know what a sub is, but you think they are trying to describe an imitation HOAGIE. You aren't a bandwagon Sixers fan you loved them when they sucked, and before they had A.I. You go to The Gallery or South Street in the summer time just to chill. You have the pizza place on speed dial. You actually get these jokes and pass them on to other friends from Philadelphia.

Sorry for the lack of posts as of late. Things in lab have been a bit crazy to say the least. I was also getting my place fixed up as I had an inspection of my apartment as the management company higher ups were doing a walk though to see how the repairs on my apartment have been going, and trust me, there have been a lot of them as of late. I thought that it would be good idea to do my (at least) twice a year clean sweep in case I do decide to move to another place. I am also trying not to lose my mind with Andrea’s wedding coming up. As always with weddings - there is way too much to be done so that everything will be perfect and never enough time to get it all done. When I was helping to get her bridal shower ready a few weekends ago, her father and I were talking about no matter how much you plan and or do, things always go wrong. Usually everyone is lucky enough that they are just minor things, but the key to the day is to make sure that no one loses it and ruin
es the whole day. Just as long as the bride and groom make it and there is someone there to marry them – that is all that matters! That and remembering to breathe.